PLANT SCIENCE BULLETIN

A Publication of the Botanical Society of America, Inc.

June 1971 Vol. 17 No. 2

Isolation and Culture of Plant Protoplasts I. K. Vasil and Vimhi Vasil.   14
The Doctor of Arts in Botany   16
Editor's Notes   17

News and Announcements
First International Congress of Systematic and Evolutionary Biology   17
Taxonomy and Phytogeography of Higher Plants in Relation to Evolution   17
Eunice Rockwell Oberly Memorial Award   17
Field Biology on Nantucket Island—Massachusetts   17
American Journal of Botany Reprint Policy   18
Agronomists Announce Meeting   18
The Second National Biological Congress   18
University of Montana Biological Station Program   19
Dr. John Walton, 1895-1971   19
Personalia   19

Book Reviews
Plant Agriculture J. Janick, R. W. Schery, F. W. Woods, and V. W. Ruttan 19
The Transport of Plant Hormones Y. Vardar   20
Papers in Plant Physiology W. S. Hillman   20

14

Isolation and Culture of Plant Protoplasts

I. K. Vasil and Vimta Vasil
Departments of Botany, and Agronomy
University of Florida, Gainesville, Florida

Within recent years techniques have been developed to isolate viable plant protoplasts in large numbers from higher plant cells by removing the cell wall with the help of enzymes. It has thus become possible to do a variety of experiments and manipulations with the plant protoplast which had so far been out of reach of botanists. The knowledge gained from the work on plant protoplasts during the last few years is of far-reaching theoretical and practical importance. It is the purpose of this article to bring this information and the possible future uses of isolated and viable plant protoplasts to the attention of a wide spectrum of botanists in the hope that the techniques of the isolation and culture of plant protoplasts can be used to probe various aspects of growth and differentiation in plants.

The cell wall around the plant protoplast is required not only for the proper physiological functioning of the plant cell system, but also for providing the necessary mechanical strength to plants which normally lack skeletal structure and organization. In addition, the wall of plant cells is of considerable historical, cultural and economic importance to man in the form of cotton, flax, or jute and other vegetable fibers, in the manufacture of finer grades of paper, as source of timber, lumber, etc. The presence of a rigid cell wall is, however, of considerable disadvantage to those interested in conducting a variety of experiments involving plant, cells and tissues for studies on nucleo-cytoplasmic interaction, the role of the nucleus and or the cytoplasm in controlling various phases of differentiation and growth, somatic hybridization, and in many areas of plant physiology, plant pathology, morphogenesis and genetics.

Animal cells, which lack a cell wall. have been used extensively with remarkable success for studies involving nuclear transplants and cell fusion (Ephrussi & Weiss, 1965; Harris, 1970; Harris et of., 1966: Ebert & Sussex, 1970). Also, bacterial (Weibull. 1958) and fungal (Bachmann & Bonner, 1959) protoplasts have proved to be of unique value in morphological, biochemical and genetic studies microorganisms. Another plant used widely for similar studies, thanks to the classic studies of Hammer-ling and later of Bracket, is the unicellular alga Acetabularia, which can be grown easily in the laboratory, has a vulnerable nucleus located in one corner of the highly differentiated and large cell, and has an exceptionally good ability for regeneration after surgical treatments.

Methodology. Mechanical methods such as microdissection. stripcutting and partial homogenization have been used from the end of the 19th century to obtain a few viable protoplasts from higher plant tissues. These protoplasts can be maintained in salt solutions for several days. In 1960. Cocking at the University of Nottingham. developed a technique for the isolation of very large numbers of plant protoplasts. This method involved the enzvmic degradation of the cell wall by cellulase from M yrotlreciunr cerrucaria. Similar forms of cellulase have since been used to isolate protoplasts from root, cotyledon, leaf. coleoptile, fruit and other tissues of plants (Cocking, 1960; Gregory & Cocking, 1961 1965; Ruesink & Thimann, 1965, 1966). Others have used pectinase followed by eelhrlase, a mixture of pectinase and cellulase (from Triehoderma virile), or partially purified enzymes, with improved results (Takebe et al., 1968; Schenk & Hildebrandt, 1969; Kao et al., 1970; Nagata & Takebe, 1970; Power & Cocking, 1970). Mixtures of pectinase and cellulase separate cells and degrade their cell walls simultaneously and thus can be used for obtaining very large quantities of intact and viable protoplasts.

Effects of Auxins. Isolated plant protoplasts provide a valuable system for studying the effect of auxins on growth and metabolism in the absence of any interfering cell wall effects. Protoplasts grown in culture and exposed to physiologically active concentrations of auxins like IAA and 2,4-D, often show increased vacuolation and eyclosis, rapid water uptake, and progressive rates of bursting. No such response is seen in cultures of protoplasts in media containing IAA together with the anti-auxin transcinnamic acid, indicating that these responses are induced specifically 1w the auxin. The rapid and dramatic bursting effect of IAA and 2,4-D on plants protoplasts points to an auxin-induced change in the permeability of the protoplast membrane system. It has also been shown that isolated protoplasts show a positive response to growth substances like IAA (and ultimately burst) while isolated vacuoles from similar protoplasts do not show any such response (Gregory & Cocking, 1966). The site of action of IAA for such a response is the plasmalemma, where the auxin facilitates an increased uptake of solutes which is followed by an osmotic water uptake resulting in bursting, as the restraining influence of the cell wall is absent in this case. Such effects of auxins on membrane permeability probably play an important role in the rapid elongation of the cell wall (Power & Cocking, 1970; van Steveninck, 1965) and may also affect the pattern of protein and nucleic acid synthesis (Davis et al., 1968).

Pinocytosis. The occurrence of pinocytosis in plant cells has been suggested by many authors to explain the up-take and transport of various substances, including virus particles. A direct and substantive proof of pinocytotic

 

PLANT SCIENCE BULLETIN
ROBERT W. LONG, Editor Life Science Bldg. 174 University of South Florida Tampa, Florida 33620
EDITORIAL BOARD Harlan P. Banks, Cornell University Sydney S. Greenfield, Rutgers University Adolph Hecht, Washington State University William L. Stern, University of Maryland Erich Steiner, University of Michigan
December 1971   Volume 17	Number Two

Changes of Address: Notify the Treasurer of the Botanical Society of America, Inc., Dr. Theodore Delevoryas, Department of Biology, Yale University, New Haven, Connecticut.

Subscriptions for libraries and persons not members of the Botanical Society of America are obtainable at the rate of $4.00 a year. Send orders with checks payable to "Botanical Society of America, Inc." to the Treasurer.

Material submitted for publication should be type-written double-spaced, and sent in duplicate to the Editor. Copy should follow the style of recent issues of the Bulletin.

Microfilms of Plant Science Bulletin are available from University Microfilms, 300 North Zeeb Road, Ann Arbor, Michigan 48106.

The Plant Science Bulletin is published quarterly at the University of South Florida 33620. Application to mail at second class postage rates is pending at Tampa, Florida.

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uptake of polystrene latex particles (Mayo & Cocking, 1969). ferritin and tobacco mosaic virus particles (Power

• Cocking, 1970; Cocking, 1966, 1970; Aoki & Takebc, 1969; Takebe & Otsuki, 1969) has been provided by using isolated plant protoplasts. It has also been shown by similar experiments using the differential staining effects of phosphotungstic acid on the plasma membrane that certain regions of the plasmalemma which take up the stain have a distinct and different chemical composition than the unstained areas of the plasmalemma, and all other organelle membranes including the tonoplast (Mayo & Cocking, 1969). The presence of vesicles in the cytoplasm with comparable staining reaction suggests that these vesicles have arisen by infoldings of the plasmalemma, and the detection of exogenously supplied ferritin in such vesicles establishes that these are pinocytotic vesicles. Isolated plant protoplasts have to be maintained in suitable but complex nutrient media often containing as much as 20n sucrose. In view of the fact that pinocytosis can be induced or inhibited liv a variety of substances, it is not vet clear whether the extent of pinocytosis observed in isolated protoplasts is close to or greatly in excess of that in the normal plant cell.

Cell Wall Regeneration in Protoplasts. Regeneration of cell walls by isolated protoplasts of fungi (Bachmann & Bonner. 1959). yeasts (Necas. 1965). and higher plant cells ( Nagata & Takebe, 1970; Pojnar et al., 1967; Mishra

• Colvin, 1969) has been demonstrated, and the importance of such systems for studying the mechanism and chomistry of cell wall deposition is quite obvious. Mishra

• Colvin (1969) have shown with the help of electron microscopic and x-ray diffraction studies that the newly-formed cell wall around isolated tomato-fruit portoplasts is not a typical plant cell wall and the origin and composition of its various layers are not vet known.

Cell wall regeneration occurs soon after the protoplasts are removed from the pectinase-ccllulase solution and seems to depend on the presence of the nucleus, as enucleate sub-protoplasts fail to regenerate a cell wall. Re-generation usually results in the formation of single spherical cells but cell aggregates can be obtained if protoplasts are kept in contact with each other during cell wall re-generation (Pojnar & Cocking, 1968).

Cell Dicision in Reconstituted Protopla.sts. One (if the difficulties often encountered in the culture of plant protoplasts has been the apparent inability of the protoplasts to undergo mitotic divisions. One to two nuclear divisions have been observed in protoplasts of Haplopappus grracilis Ericksson & (onasson, 1969). Recently. Nagata & Takebe (1970) and Tao et al. (1970) were not only able to re-generate cell walls in protoplasts obtained from tobacco leaf mesophvll cells and soybean cells in suspension culture. but were also able to induce repeated mitotic divisions in the newly reconstituted cells resulting in the formation of groups of eight or more cells. Isolated moss protoplasts readily regenerate cell wall in culture. and then form protonemata and whole plants (Binding, 19661.

Fusion of Isolated Protoplasts. One of the most important and far-reaching aspects of the development of precise techniques to isolate and successfully culture plant protoplasts is the fact that isolated protoplasts fuse to form he tend<arvous under suitable emu-lit-ions (Power et al.. 1970: Cocking. 1971). 'Washing the protoplasts with (1.25M sodium nitrate seems to induce fusion, probably by affecting the electrical and physiological properties of the plasmalemma. The presence of an inactivated virus is not required for bringing about the fusion of plant protoplasts although that is a pre-requisite for obtaining fusion of animal cells. The first sign of impending fusion between protoplasts is the formation of extremely fine tubular outgrowths from the surface of the plasmalemma; similar outgrowths are formed during cell wall regeneration by protoplasts (Cocking, 1968). Interspecific heterokarvons also have been obtained from the fusion of maize and oat protoplasts (Power et al., 1970). It appears that fusion of protoplasts from any two plants can be achieved as long as the protoplasts are healthy, nucleate, and meet certain requirements in terms of the distribution and arrangement of cytoplasm within the plasmalemma. No cell wall regeneration or nuclear division has been reported in fused protoplasts of inter- or intra-specific origin. Regeneration of a cell wall around the heterokarvon and fusion of nuclei within the heterokarvon during simultaneous mitotic divisions would give rise to a true hybrid plant cell. Such a cell could then be grown by known techniques to obtain callus and then a whole plant (V. Vasil & IfiIdebrandt, 1965a, in 1967; I. K. Vasil & Hildebrandt, I966a, b): these plants in most cases will be allotetraploids. Somatic hybridization between species which can not be mated by conventional methods may thus be achieved through fusion of plant protoplasts. However. it is perhaps too speculative at this time to claim that somatic hybrids can he obtained by this technique from widely separated families or groups of plants.

One of the important applications of the technique of isolation and culture of plant protoplasts could be the possibility of making inter- and intra-specific nuclear or cell organelle (plastids. mitochondria. dictvosomes, ribosome. etc.) transplants for understanding problems of the control of growth. differentiation and morphogenesis.

Reiter;r;Nc is

Aoki. S.. and Takebe, 1. 1969. Infection of tobacco mesaphyil protoplasts by toh;cco mosaic ribonucleic. acid. Virology 39:439-448.

Bachmann, B. J., & Bonner, D. M. 1959. Protoplasts from Neurospora crasa. 1. Bact. 78:550-556.

13indine. H. 1966. Regeneration nod Verschmclzung nacktcr

Laubmoosprotoplasten. Z. Pflanzenphysiol. 55:305. Cocking. E. C. 1960. A method for the isolation of plant

protoplasts and vacuoles. Nature 187:927-929.

Cocking. E. C. 1966. An electron microscopic study of the initial stages of infection of isolated tomato fruit protoplasts by tobacco mosaic virus. Planta 68:206-214.

Cocking., E. C. 1968. The action of 3-indoleacetic acid on

isolated protoplasts. In "Biochemistry and Physiology of

Plant Growth Substances." F. \i'fightman & G. Setterfield

(eds.). Runge Press, Ottawa.

Cocking.. E. C. 1970. Virus uptake, cell wall regeneration, and virus multiplication in isolated plant protoplasts. Int. Rev. Cytol. 28:89-124.

Cocking. E. C. 1971. Fusion of isolated protoplasts: a first step towards the somatic hybridization of plants. In Proc. IInd Int. Conf. Plant Tissue Culture. C.N.R.S., Paris (In Press).

Da ies. 1). I).. Patterson. 13. P.. & Trewavas. A. J. 1968. Stu-

dies on the mechanism of action of indole-acetic acid, In

"Plant Growth Regulators." S.C.I. Monograph No. 31. Ebert. J. D. & Sussex. I. M. 1970. Interacting Systems in

Development. 2nd. ed. IIolt, Rinehart & Winston. New

York.

Ephrussi, B., & Weiss. M. C. 1965. Interspecific hybridization
of somatic cells. Proc. Nat. Acad. Sci. 53:1040-1042,

Eriksson, T., & ]onasson, K. 1969. Nuclear division in isolated protoplasts from cells of higher plants grown in vitro. Planta 89:85-89.

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Gregory, D. NV., & Cocking, E. C. 1963. The use of polygalacturonase for the isolation of plant protoplasts and vacuoles. Biocheun. J. 88:40.

Gregory, D. W., & Cocking, E. C. 1965. The large-scale isolation of protoplasts from immature tomato fruits. J. Cell Biol. 24:143-146.

Gregory, D. 'W., & Cocking, E. C. 1966. Studies on isolated protoplasts and vacuoles. II. The action of growth sub-stances. J. Exp. Bot. 17:68-77.

Harris, II. 1970. Cell Fusion. Harvard Univ. Press. Cam-bridge, Mass.

Harris, H., Watkins, J. F., Ford, C. E., & Schoefl, G. I. 1966. Artificial heterokarvons of animal cells from different species.   J. Cell Sci. 1:1-30.

Kao, K. N., Keller, W. A., & Miller, R. A. 1970. Cell division in newly formed cells from protoplasts of soybean. Exp. Cell Res. 62:338-340.

Mayo, M. A., & Cocking, E. C. 1969. Detection of pinocvtic activity using selective staining with phosphotnngstic acid. Protoplasma 68:231-236.

Mishra, A. K.. & Colvin, J. R. 1969. The formulation of wall-like envelopes by isolated tomato-fruit protoplasts. Protoplasma 67:295-305.

Nagata, T., & Takebe, I. 1970. Cell wall regeneration and cell division in isolated tobacco mesophyll protoplasts. Planta 92:301-308.

Necas, O. 1965. The mechanism of regeneration of yeast protoplasts. II. Formation of the cell wall de noro. Folia Biol. (Prague) 11:97-102.

Pojnar, E., & Cocking, E. C. 1968. Formation of cell aggregates by regenerating isolated tomato-fruit protoplasts. Nature 218:289.

Pojnar, E., Willison, J.H.M., & Cocking, E. C. 1967. Cell-wall regeneration by isolated tomato-fruit protoplasts. Protoplasma 64:460-480.

Power, J. B., & Cocking, E. C. 1970. Isolation of leaf protoplasts: macromolecule uptake and growth substance response. J. Exp. Bot. 21:64-70.

Power, J. B., Cummins, S. E., & Cocking, E. C. 1970. Fusion of isolated plant protoplasts. Nature 225:1016-10]8.

Ruesink, A. W., & Thimann, K. V. 196.5. Protoplasts from the Arena coleoptile. Proc. Nat. Acad. Sci. 54:56-64.

Ruesink, A. W., & Thimann, K. V. 1966. Protoplasts: preparation from higher plants. Science 154:280-281.

Schenk, R. U., & Hildebrandt, A. C. 1969. Production of protoplasts from plant cells in liquid culture using purified commercial cellulascs. Crop. Sci. 9:629-631.

Takebe, I., Otsuki, Y., & Aoki, S. 1968. Isolation of tobacco mesophvll cells in intact and active state. Plant & Cell Physiol. 9:115-124.

van Steveninck. 1965. Effect of indolyl-3-acetic acid on the permeability of membranes in storage tissue. Nature 205:83-84.

Takebe, I., and Otsuki, T. 1969. Infection of tobacco mesopyll protoplasts by tobacco mosaic vines. Proc. Nat. Acad. Sci. 64:84:3-848.

Vasil, I. K., & Hildebrandt, A. C. 1966a. Variations of morphogenetic behaviour in plant tissue cultures. I. Cichorium endicia. Amer. J. Bot. 53:860-869.

Vasil, I. K., & Hildebrandt, A. C. 1966b. Variations of morphogcnetic behaviour in plant tissue cultures. II. Petro-.selinum hortense. Amer. J. Bot. 53:869-874.

Vasil, V., & Hildebrandt, A. C. 1965a. Growth and tissue formation from single, isolated tobacco cells in microculture. Science 147:1454-1455.

Vasil, V., & Hildebrandt, A. C. 1965b. Differentiation of tobacco plants from single, isolated cells in microcultures. Science 150:889-892.

Vasil, V., & Hildebrandt, A. C. 1967. Further studies on the growth and differentiation of single, isolated cells of tobacco in vitro. Planta 75:139-151.

\Vcibull, C. 1958. Bacterial protoplasts. Ann. Rev. Micro-biol. 12:1-26.

The Doctor of Arts in Botany

With increasing emphasis being placed upon ecology, environmental pollution, and the conservation of natural and human resources, many institutions are restructuring their biological programs to prepare students for active and productive careers in these fields. The Claremont Graduate School, Claremont, California has recently approved a new Doctor of Arts degree program in the Biological Sciences, with emphasis on Botany. The Doctor of Arts program is presented as a way of better training botanists for participation in biology programs in state, liberal arts, and community colleges. The program would be tailored to the needs and interests of the individual student and would be formulated to give him a broadly based background in the biological and environmental sciences with major emphasis on plant science.

The Ph.D. program would remain what it has been, a program for research oriented students who will most likely find employment in universities where emphasis is shared between teaching and research.

To the limits of the faculty's ability, the two programs would remain equal as to qualifications for entrance, the quality of performance required of the student. The Doc-tor of Arts would not be considered a second-rate Ph.D. or a substitute for the Ph.D. The two would be considered equal but with different goals.

The academic requirements for admission to the Doctor of Arts program will be the same as for admission to the Ph.D. program. Students holding an M.S. degree from an acceptable institution would be allowed to transfer up to 24 units of class work. The decision on the suitability of the courses for transfer and the number of units allowed will be made by the faculty.

In recent years the graduate student population in botany has varied from 7 to 11. It is not anticipated that this number will be greatly increased in the foreseeable future. If a Doctor of Arts program is given it is expected that there will be no more than one of two students en-rolled in it at any one time and that the total number of students in botany will be no more than it would be with-out the program.

It is not anticipated that there will be any difficulty in recruiting students and already there are two who have indicated that they would like to enroll this coming fall if such a program is available. One has a M.S. and since 1967 has been an instructor in biology at Youngstown State University, Ohio. The other will receive his B.A. this June from the University of Maryland and has indicated an interest in preparing for college teaching. One advanced and one beginning student would give a desirable spread at the beginning of the program.

Full-time students in botany who hold graduate trainee-ships now enroll for two classes each semester from the botany curriculum. In addition, they enroll for independent research, tutorial reading, master's thesis or doctoral study (as appropriate). Students in the Doctor of Arts program would also enroll for two classes from the botanical curriculum and in addition would elect one course each semester from the zoological and microbiological curricula. Some time during their program they would also enroll for two courses in Education. The first year would involve 24 units of class work and seminars; the second year would involve 24 units of class work and seminars. The third year would be a teaching internship, also for 24 units.

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A qualifving examination shall be taken not later than the end of the second year of study.

Summer Programs: During the first two summers, field experience would be gained by enrolling for studies at one or more biological stations or laboratories. The choice of the station, or laboratory, would depend upon the student's interests and needs. Examples of acceptable institutions are: Santa Catalina Marine Biological Laboratory, Hopkins Marine Station, University of Michigan Biological Station, the Smithsonian Institution Biological Station, Barro Colorado Island. Canal Zone or biological program. Office of Tropical Studies, San Jose, Costa Rica.

One foreign language is required and in addition one of the following: a second foreign language; a suitable course in statistics or computer science; or Advanced Techniques in Botanical Research.

Each candidate for the Doctor of Arts degree would be required to present a review paper on some suitable subject. The subject and scope of the paper to be approved by a faculty committee in consultation with the student.

Editor's Notes

The editor is pleased to report that former editor Dr. Harry J. Fuller is very much alive and presently is residing at The Americana Nursing Center in Urbana, Illinois. Sincere apologies are extended to both Dr. Fuller and to his friends for referring to the "late" Dr. Fuller in the last issue of PSB.

Reaction to the new format has been very good, and apparently the BULLETIN is reaching the membership in good condition despite being mailed "flat". Suggestions that will result in an improved PSB are always welcome, as well as contributed papers. news_ announcements, and the like.

News & Announcements

First International Congress of Systematic and Evoluntionary Biology

The Society of Systematic Zoology and the International Association for Plant Taxonomy have joined forces to develop this first opportunity for botanical%zoological inter-action at the international level. The University of Colorado (Boulder, Colorado) has extended a gracious invitation to meet on that campus August 4-11, 1973. The diversity of ecological situations in the surrounding country-side makes this one of the most attractive sites in North America, both aesthetically and scientifically. The presence of experienced, enthusiastic biologists on that campus also provides an indispensable ingredient for the success of this Congress.

Program plans at this point encompass interdisciplinary symposia and contributed paper sessions. The botanists will not convene a nomenclatural section but a zoological one on this subiect is anticipated. In the next few months the outline of the program and other activities will begin to take form. All suggestions will be gratefully received. carefully considered, and as many adopted as practical or feasible. Correspondence may he addressed to the Secretarv: Dr. James L. Reveal. Department of Botany, Universitv of Maryland. College Park. Maryland 20740.

Taxonomy and Phytogeography of Higher Plants in Relation to Evolution

This conference has been organized by Professor D. H. Valentine on behalf of the Linnean Society of London, the Botanical Society of the British Isles and I. O. P. B. It will be held at the Botany Department, University of Manchester, England on 9th-11th September, 1971. The programme will be grouped into sections covering the following topics:—I. Floristic elements. 2. Major geographical disjunctions in relation to evolution and migration—(a) Vicarious species; (b) Migration. 3. Endemism—(a) Evolution of island floras; (b) Special topics. 4. Geographical evolution in general of special interest. Among those from whom provisional acceptances to speak have been received are:—Prof. J. Kornas (Krakow), Prof. H. Ilara (Tokyo), Prof. 7'. W. Bocher (Copenhagen), Dr. D. M. Moore and Dr. D. Bramwell (Reading), Dr. D. F. Cutler (Kew), Prof. O. T. Solbrig (Harvard), Dr. W. Grouter (Geneva), Prof. H. Lewis (Los Angeles, California), Plot. C. Favarger (Neuchatel), Prof. C. van Steenis (Leiden), Dr. N. Jardine and Dr. S. M. Walters (Cambridge), Dr. A. Strid (Lund), Dr. H. G. Baker (Berkeley, California) and Professor A. R. Clapham, F.R.S.

There will he a field excursion on Sunday, 12th September 1971. The Local Secretary is Dr. C. A. Stace, Botany Department, The University, Manchester M 13 9PL, England, from whom further particulars may be obtained.

Eunice Rockwell Oberly Memorial Award

A biennial award has been established, to be given in odd-numbered years. and consisting of a citation and a cash award from the income of the Oberly Memorial Fund. It is to be administered by the Agricultural and Biological Sciences Subsection, Subject Specialists Section, Association of Colleges and Research Libraries of the American Library Association.

The award will be made to the American citizen who compiles the best bibliography in the field of agriculture or in one of the related sciences in the two-year period preceding the year in which the award is made. The fund was established by colleagues in memory of Eunice Rock-well Oberly. Additional information regarding this award can be obtained from Mr. Fleming Bennett, University of Florida Libraries, Gainesville, Fla. 32601.

Field Biology on Nantucket Island - Massachusetts

The Biology Department of the University of Massachusetts - Boston will offer a course in field biology at its research facility on Nantucket Island from July 25 through September 1 this summer. The course is designed for advanced undergraduates and offers six credits. Each student is required to conceive of, carry out and write un an original research project in field biology. A number of habitats allowing, study of both plants and animals are available at or near the Research Center. These include salt marsh and estuarine areas, shallow coastal waters, sand dune. moorland and upland scrub. Limited living facilities are available at modest cost. Students desiring more detailed information concerning admission and con-duct of the course should contact Wesleu N. Tiff netl Jr., Biologv Department. University of Massachusetts—Boston, 100 Arlington St., Boston, Mass. 02116.

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American Journal of Botany Reprint Policy

Owing to increasing costs and decreasing revenues, Dr. Lawrence J. Crockett, Business Manager, American Journal of Botany, regrets to announce that the very liberal rule that everybody who publishes in the journal receives the first 100 reprints free must be changed. Beginning with the August issue, only those who are paying the voluntary page charge will get the reprints free.

Hopefully, members of the Society will understand why this change is necessary. Our membership dues are very low in comparison to other similar scientific societies. It has been possible for a member who published two articles in one year to get back as much as S30.00 on his $10.00 membership fee. While finances were rosy, this could be tolerated. but with science and economics being what they are today, the Society can no longer grant this gift.

Agronomists Announce Meeting

The American Society of Agronomy and its two sister societies, Crop Science Society of America and Soil Science Society of America, have announced preliminary plans for their 1971 annual meeting to be held in New York City, August 15-20. Theme of this year's meeting is "Agronomy and Environmental Quality." This meeting has the potential of being the greatest agronomic gathering in the Societies' history.

Through the special symposia that are planned, the Societies will have the opportunity of speaking to scientists in related disciplines, the layman public and the press about the position of the agronomic sciences on the environmental issues of today. Some of the symposia to be held are: Breeding Crops for Resistance to Insects; Fate and Economic Impact of Herbicides in the Environment; and Sediments and Water Quality.

Dr. Norman E. Borlaug, who has been called the father of the green revolution for his invaluable work in developing high-yield wheat strains, is to be the guest of honor and featured speaker at a combined session of the Crop Science Society of America and Soil Science Society of America. This session will he held at 8:00 p.m., Monday. August 16, as a feature event of the annual meeting of the American Society of Agronom and these two societies.

Dr, Borlaug's topic will be "Potentials and Limitations of Improving Crop Varieties to Meet World Food Needs." He has devoted his talents and energies toward improving and expanding crop yields and duality since 1944 and feels the greatest problem mankind must solve is development of ways and means of feeding the expanding world population.

Because of his untiring efforts for humanity, Dr. Borlaug has been awarded the Nobel Prize for Peace. Of this, Dr. Borlaug, has said. "It is not my prize, but a prize for agriculture."

The Second National Biological Congress

The Second National Biological Congress will be held in one of the most delightful spots in the United States. Miami Beach. It begins on 23 October and continues through 26 October 1971. There will he 3 days of symposia, partly on very general topics and partly on more specific problems. There will also he social events, a few major speeches, and biological field trips.

The theme is "Man and Environment II," and will focus specifically on: What are the problems posed by our environment? What success have we had so far in dealing with them? and What is the outlook?

The morning programs will center on three practical aspects of the work of biologists in relation to the environment:

BIOLOGISTS AS PLANNERS

Ecological Engineering

organized by Athelstan Spilhaus

Problems of Developing the New World

To he announced

Ecology & the Planning of Future Cities

organized by Keith Hay

Agriculture in an Urban Society

organized by Edward J. Ryder

Scientific Aspects of Human Population Control To be announced

BIOLOGISTS AS OBSERVERS

Radiation: Its Control & Future Dangers

organized by Alexander Hollaender

Primary Productivity

organized by Robert Whittaker and Gene Likens Our Mobile Earth

organized by William Benson

World Trends in Epidemic Diseases

organized by Marshall Laird

World Trends in our Environment

organized by Thomas F. Malone

BIOLOGISTS AS PROBLEM SOLVERS Problems of Sewage

organized by Gilbert Levin

Weather Modification: Ecological Opportunities and Environmental Problems

organized by Charles Cooper & Frank Eden (Cosponsored by the American Meteorological Society) The International Biological Program

organized by \V. Frank Blair

Strategy of Approaching World Protein Food Problems organized by Aaron \I. Altschul

Past, Present. and Future of the Everglades

organized by Howard Teas

The New Knowledge of Auto-Immunity

To be announced

The program will also include a special symposium to celebrate the Bicentenary of the Discocery of Photosynthesis. and the Atwater Memorial Lecture Award, presented to a leading scientist who will speak on problems of nutrition and the world's food supply.

The afternoon programs are being planned by the biological societies. At this time. these include American Society of Biological Chemists, American Society for Horticultural Science. American Society of Parasitologists, American Society of Zoologists, Society of Invertebrate Pathology. Society for the Study of Evolution, The Nature Conservancy. National Wildlife Federation, The Wildlife Society, The Teratology Society. American Society for Experimental Pathology, and Federation of American Societies for Experimental Biology. Mane of these societies will have joint programs.

The last day of the Congress has been set aside for biological field trips. South Florida is an extremely interesting area biologically. and the weather in October is ideal. Visits will include Everglades National Park. Fair-child Tropical Garden, Plant Introduction Station, Pigeon Key Marine Station, Scaquarium. and the Lerner Marine Laboratory at Bimini.

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For further information about the Congress, and for registration and housing arrangements. Society members should write to the National Biological Congress, American Institute of Biological Sciences, :3900 Wisconsin Ave.. N. W., Washington, D. C. 20016.

University of Montana Biological Station Program

The Biological Station, a unit of the Summer Session of the University of Montana, has announced its program for 1971. The Station, located at Yellow Bav, Flathead Lake, Bigfork, Montana will offer six courses in botanical science during the summer session June 27-August 21. Systematic Botny, Phycology, Morphology, Problems in Taxonomy. Mycology, and General Ecology will be given together with selected courses in Zoology. The faculty will be Drs. John Tibbs. Director. Arden Gaofine, Gerald \V. Prescott. B. L. Turner, Robert L. Fisher, Edward E. C. Clebsch, Orson K. Miller, Benjamin A. Foote, and James J. O'Toole. Persons interested in the program, or in opportunities for study and research at the Station should write to Dr. Tibbs, Biological Station, University of Montana, Missoula. Montana 59801, or care of the summer mailing address University of Montana Biological Station, Bigfork. Montana 5991 I.

Dr. John Walton, 1895-1971

John Walton, rcgius professor of botany at the University of Glasgow from 1930 until 1962, died on February 13th in Dundee, Scotland.

A Fellow of the Royal Society of Edinburgh and a Corresponding Member of the Botanical Society of America, Professor Walton was internationally famous for his studies of Paleozoic plants. His influence has been felt, knowingly or unknowingly, by every paleobotanist who ever made a cellulose acetate peel or a transfer preparation of a fossil specimen because he originated both of these basic and indispensihle techniques. Indeed, progress in the field of paleobotanv has been so greatly facilitated by their universal application that, without any discredit to his mans' important botanical contributions. they can be considered Professor Walton's most significant contributions to science.

Professor Walton was horn in London, and educated at Edinburgh and Cambridge. In 1921 he was botanist with the first Oxford expedition to Spitzbergen. Following his retirement he was given the honorary appointment. Dean of Faculties, by the University of Glasgow. Ile received Doctor of Science degrees from Cambridge and Manchester, honorary Doctor of Science degrees from Montpellier and Lille, and an honorary Doctor of Laws degree from McMaster.

In addition to his interests in morphology and paleobotany he had a deep concern for nature and its conservation. This interest, which persisted throughout his life-time, is reflected in certain positions he held. He was President of the Glasgow Tree Lover's Association and the Scottish Youth Hostels Association, a forestry commissioner. and a member of the Scottish Committee of the Nature Conservancy.

Hi.s interests also encompassed the fine arts, and he served as honorary curator of the University of Glasgow's art collections.

Professor Walton visited North America on several occasions, and will be remembered personally by his many American and Canadian friends amongst whom arc several who studied under him as exchange students or post-doctoral fellows.

Charles B. Beck

Personalia

Dr. Anton Lang, director of the AEC Plant Research Station at Michigan State University, has been appointed chairman of the NAS/NRC group studying the effects and dangers of herbicides and defoliation in Vietnam.

Dr. Barbara McClintock, Carnegie Institution of Washington, was among the recipients of the 1970 National Medal of Science. the highest award of the federal government for distinguished achievement in science, mathematics, and engineering. She was recognized for her re-search in establishing the relations between inherited character of plants.

Two Colorado botanists have received a National Science Foundation grant to conduct a study of the growth and development of plants common to the Rocky Mountain tundra regions of Boulder county and to various arctic regions of the world. Dr. Erik K. Ronde, University of Colorado, and Mrs. Maxine Foreman, Denver Community College. will conduct the studies at the University, and at the Institute of Arctic and Alpine Research Station during the 1971-72 year. Plants will be grown at the expermiental gardens ranging in altitude from 5500 feet at Boulder to 12.300 feet at Niwot Range, and comparisons will be made with results from other tundra sites, such as those in Russia, Norway and Alaska. The research is part of the .5-year, 60-nation International Biological Program (IBP).

Dr. Arthur II. Westing, Department of Biology, Wind-ham College, Putney. Vermont 05346, is conducting re-search on the ecological impact of bomb craters. He would he interested in corresponding with anyone who has any information on the literature of this subject.

Book Reviews

JaNres, j., It. W. Scrur:ay, F. W. Woos, and V. W. RUT-

TAN. Plant Agriculture.   Readings from Scientific

American. W. H. Freeman and Co., San Francisco,

1970. 246 pp., illus. $10.00 (paperbound $4.95). This is an interesting and useful book. Although it consists of twenty-five separate articles that appeared in Scientific American between 1950 and 1969, the book has logical coherence due to intelligent selection and arrangement. The articles are grouped into five sections: 1. Agricultural Beginnings (old and new world). 2. Plant Growth and Development (photosynthesis, control of growth, flowering, fruiting). 3. Plant Environment (light, soil, water and climate), 4. Production Technology (desert reclamation, hybrid crops, fertilizers, pesticides. harvesting and processing) and 5. Food Needs and Potentials (various aspects of world food and population problems). Each section is preceded by a coordinating introduction. Biographical notes on the authors, bibliographies for each article and general index are included at the end of the hook.

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The articles are all written by experts in the several fields. They are highly informative, profusely and beautifully illustrated, and in a clear, concise and interesting manner give the results of scientific research in the broad range of areas discussed. Of course, reprinting articles as they were originally published over a span of years leaves some of them not entirely up to date, but this is not a serious handicap, The interested student can be encouraged to pursue recent work in any area that stimulates his interest.

This book contains some of the most fascinating aspects of botany and agriculture and demonstrates the vital importance of plants and plant studies to human life and civilization. It should be useful as collateral reading in general biology and botany courses as well as in courses in economic botany and others dealing with resources and population problems. High school and college teachers of biology would profit by reading this book. It should help them and their students to realize that plants, plant science and technology are as interesting and important as any-thing man studies, and are, moreover, the most "relevant" studies of our time. This is an attractive, interesting and convenient compilation. It deserves to be widely read.

Sydney S. Greenfield

VARDAR, Y. (Editor). The Transport of Plant Hormones. North-Holland Publishing Company, Amsterdam. 1968. 457 pages. $23.00, from john Wiley & Sons, New York.

This book, a proceedings of the NATO Ege University Summer Institute held in Izmir, Turkey in 1967, consists of 25 papers presented by a fairly impressive array of authorities. The subject matter dealt with is for the most part auxin transport and its effects on correlation phenomena.

Joseph Scheibe

HILLMAN. W. S. (Compiler). Papers in Plant Physiology. Holt, Rinehart and Winston. Inc., N. I. 1970. 591 pp. S6.95.

Anyone who intends to use this collection should read the preface; it is well written and informative. Students in particular should be referred to the note on reading scientific papers. The objective of the collection is to assemble under one cover a few of the recent, most readable and most interesting scientific research papers which reflect the breadth of plant physiology and illustrate some of the current trends in the field. No particular effort

has been made to include classical papers rather those which are especially well written and fairly brief. Indeed, as the compiler points out, inclusion of a paper "does not necessarily imply that it is highly original or fundamental in its area" and "an equally appropriate collection could be made without including any of the papers chosen". It does not seem appropriate to consider merits and demerits of individual papers in this review: most of the papers selected with a couple of possible exceptions are of high scientific quality. The compiler himself notes that inclusion in this volume is neither a guarantee of quality nor any assurance the work will stand the test of time.

The intended use of this collection, especially for teaching purposes, is less clear. Presumably, the collection would be used by undergraduate students or even beginning graduate students in order to make it easier for the student (or the teacher) to find some good research papers. I certainly favor the idea of having undergraduate students (even first-or second-year students who are interested) read a few original research papers. These can convey the excitement of science and a feeling for the process of scientific inquiry; however, this collection isn't necessary to achieve that goal.

I think it would be very difficult for a student to set each report into the broader perspective and see its contribution to the development of its special area. Thus, it would be very helpful for the sutdent to have some sort of introduction to each of the six sections. The compiler recognizes this shortcoming and concludes, probably correctly, that it would be impossible to produce these summaries in a brief form. If the collection had focused on six important lines of investigation. rather than trying to span the entirety of plant physiology, it might have been possible to provide a brief summary-overview for each section. Concentration on a couple of important themes would also have enabled even the uninitiated reader to observe and understand the evolution of some important principles.

In conclnsion, the job done by this collection could he achieved more economically and perhaps more effectively by a list of suggested readings at the end of each chapter in a plant physiology text (this reinforced by the instructor's encouragement to read some original papers). This would have the advantage that the text itself would help the student to see Lou each research report contributed to the development of the field.

Tarry D. Nooden

PLANT SCIENCE BULLETIN LIFE SCIENCE BUILDING UNIVERSITY OF SOUTH FLORIDA TAMPA, FLORIDA 33620